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KMID : 0614020090240010083
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Journal of Pharmaceutical Sciences (C.N.U.)
2009 Volume.24 No. 1 p.83 ~ p.87
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The Change of Spectrophotometric Characteristics of Alendronate Using the Mixture with Ferric Ion
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Song Ji-Keun
Nam Jin-Kyung
Cho Cheong-Weon
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Abstract
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Alendronate, one of bisphosphonates is widely used in the clinical treatment of systemic metabolic diseases, such as osteoporosis and Paget’s disease as well as the sequela of malignancies including hypercalcemia. However, alendronate has no chromophore, so the method of derivatization with FMOC(9-fluorenylmethyl chloroformate) in a citrate buffer(pH 11.9) using HPLC was developed. In this study, we developed UV-VIS spectrophotometric assays for availability, simplicity and cost-effectiveness of the required instrumentation. The stoichiometric ratio of alendronate to Fe^(+)³ in the chromophoric complex was determined to be 1:1.
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KEYWORD
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Alendronate, HPLC analysis, chromophore
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